of 299 · 23rd in UEFA
Projected roster. Final squads will be updated before the World Cup starts.
| Pos | Name | Detailed Pos | Club | League |
|---|---|---|---|---|
| GK | Lukáš Horníček | SC Braga | Primeira Liga | |
| GK | Matěj Kovář | PSV Eindhoven | Eredivisie | |
| GK | Jindřich Staněk | SK Slavia Prague | Czech First League | |
| DEF | Vladimír Coufal | TSG Hoffenheim | Bundesliga | |
| DEF | David Douděra | SK Slavia Prague | Czech First League | |
| DEF | Tomáš Holeš | SK Slavia Prague | Czech First League | |
| DEF | Robin Hranáč | TSG Hoffenheim | Bundesliga | |
| DEF | Štěpán Chaloupek | SK Slavia Prague | Czech First League | |
| DEF | David Jurásek | SK Slavia Prague | Czech First League | |
| DEF | Ladislav Krejčí | Wolverhampton Wanderers | Premier League | |
| DEF | Jaroslav Zelený | AC Sparta Prague | Czech First League | |
| DEF | David Zima | SK Slavia Prague | Czech First League | |
| MID | Lukáš Červ | FC Viktoria Plzeň | Czech First League | |
| MID | Vladimír Darida | FC Hradec Králové | Czech First League | |
| MID | Lukáš Provod | SK Slavia Prague | Czech First League | |
| MID | Michal Sadílek | SK Slavia Prague | Czech First League | |
| MID | Hugo Sochůrek | AC Sparta Prague | Czech First League | |
| MID | Alexandr Sojka | FC Viktoria Plzeň | Czech First League | |
| MID | Tomáš Souček | West Ham United | Premier League | |
| FWD | Adam Hložek | TSG Hoffenheim | Bundesliga | |
| FWD | Tomáš Chorý | SK Slavia Prague | Czech First League | |
| FWD | Mojmír Chytil | SK Slavia Prague | Czech First League | |
| FWD | Jan Kuchta | AC Sparta Prague | Czech First League | |
| FWD | Patrik Schick | Bayer Leverkusen | Bundesliga | |
| FWD | Pavel Šulc | Olympique Lyonnais | Ligue 1 | |
| FWD | Denis Višinský | FC Viktoria Plzeň | Czech First League |
